Mechanism of Polymerization of the Salmonella O-Antigen: Utilization of Lipid-Linked Intermediates
AUTOR(ES)
Kanegasaki, Shiro
RESUMO
Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283297Documentos Relacionados
- Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium.
- Glycoprotein Biosynthesis in Cotyledons of Pisum sativum L: Involvement of Lipid-linked Intermediates 1
- In vitro synthesis of a lipid-linked trisaccharide involved in synthesis of enterobacterial common antigen.
- In Vitro Synthesis of a Lipid-Linked Trisaccharide Involved in Synthesis of Enterobacterial Common Antigen
- Accumulation of a lipid-linked intermediate involved in enterobacterial common antigen synthesis in Salmonella typhimurium mutants lacking dTDP-glucose pyrophosphorylase.
