Membrane permeability changes associated with DNA gyrase inhibitors in Escherichia coli.
AUTOR(ES)
Dougherty, T J
RESUMO
Inhibition of DNA synthesis in Escherichia coli B/r by a DNA gyrase inhibitor results in cell death after a 50-min lag period. Examination of the cells under phase-contrast and electron microscopes revealed that they appeared to undergo plasmolysis coincident with the onset of cell death. The inhibited cells were also found to become susceptible to low levels of detergent at this time. With a fluorescent membrane probe, the level of membrane permeability was assessed and found to increase concurrently with the decrease in culture viability. Analysis of the cell envelope constituents revealed that, other than a shift in the protein/lipid ratio, the compositions of the cell membranes were unperturbed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=180219Documentos Relacionados
- Gyrase inhibitors increase the content of knotted DNA species of plasmid pBR322 in Escherichia coli.
- Effect of outer membrane permeability on chemotaxis in Escherichia coli.
- Effects of DNA gyrase inhibitors in Escherichia coli topoisomerase I mutants.
- Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
- Effect of DNA gyrase inactivation on RNA synthesis in Escherichia coli.