Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells
AUTOR(ES)
Bussell, Robert H.
RESUMO
A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 × 105. MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356626Documentos Relacionados
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