Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and Man
AUTOR(ES)
Mills, John B.
RESUMO
As a first step in our study of structure-function relationships among primate and non-primate growth hormones, human growth hormone (hGH) was subjected to the limited digestive activity of human plasmin. The lyophilized whole digest, containing less than 2% of unchanged hormone, had an average of 2.3 new amino-terminal groups per mole. The digest had the same potency as the native hormone (a) in causing weight gain in hypophysectomized rats; (b) in stimulating somatomedin production in hypophysectomized rats; (c) in stimulating upake of [3H]leucine into isolated diaphragm of hypophysectomized rats; (d) in accelerating transport of [14C]α-aminoisobutyric acid into isolated diaphragm of hypophysectomized rats; (e) in stimulating uptake of [3-0-methyl-14C]glucose by isolated adipose tissue of hypophysectomized rats; (f) in accelerating conversion of [14C]glucose to 14CO2 by isolated epididymal adipose tissue of hypophysectomized rats. The digest also caused glucosuria in partially pancreatectomized rats treated with dexamethasone.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=302563Documentos Relacionados
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