Methanobacterium thermoautotrophicum RNA Polymerase and Transcription In Vitro
AUTOR(ES)
Darcy, Trevor J.
FONTE
American Society for Microbiology
RESUMO
RNA polymerase (RNAP) purified from Methanobacterium thermoautotrophicum ΔH has been shown to initiate transcription accurately in vitro from the hmtB archaeal histone promoter with either native or recombinant forms of the M. thermoautotrophicum TATA-binding protein and transcription factor TFB. Efforts to obtain transcription initiation from hydrogen-regulated methane gene promoters were, however, unsuccessful. Two previously unrecognized archaeal RNAP subunits have been identified, and complex formation by the M. thermoautotrophicum RNAP and TFB has been demonstrated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=93948Documentos Relacionados
- Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro.
- Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum
- Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum
- TFE, an Archaeal Transcription Factor in Methanobacterium thermoautotrophicum Related to Eucaryal Transcription Factor TFIIEα
- Transcription of the ileS operon in the archaeon Methanobacterium thermoautotrophicum Marburg.
