Micro-Media Systems anaerobe panel versus broth disk method in anaerobe antimicrobial testing.
AUTOR(ES)
Polomski, J C
RESUMO
Frozen microtitration trays manufactured by Micro-Media Systems (MMS) for antimicrobial testing of anaerobic bacteria were evaluated and compared with the broth disk elution method of Virginia Polytechnic Institute (VPI) described by Wilkins and Thiel. A total of 224 clinical anaerobic isolates were tested. Susceptibility results were compared for carbenicillin, cefoxitin, chloramphenicol, clindamycin, penicillin, and tetracycline. Published procedures for the MMS and VPI methods were followed using aerobic inoculation. Growth performance in the MMS method was 74% compared with 89% in the VPI method. For each antibiotic, the MMS minimum inhibitory concentration was compared with the VPI test concentration. Of the 972 drug-organism combinations, 2.9% of the results were discrepant. Clindamycin and tetracycline accounted for 82% of the discrepancies. The MMS anaerobe susceptibility panels were easy to inoculate and interpret and compared well with the VPI broth disk susceptibility method.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272781Documentos Relacionados
- Collaborative evaluation of the micro-media systems anaerobe susceptibility panel: comparisons with reference methods and test reproducibility.
- Evaluation of the Micro-Media System for Quantitative Antimicrobial Drug Susceptibility Testing: a Collaborative Study
- Identification of Enterobacteriaceae in frozen microdilution trays prepared by Micro-Media Systems.
- Evaluation of Micro-Media Quad Panels for identification of the Enterobacteriaceae.
- Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae.
