Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization

AUTOR(ES)

Llobet-Brossa, Enric

FONTE

American Society for Microbiology

RESUMO

The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4′,6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.

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