Microcalorimetric study of the anaerobic growth of Escherichia coli: measurements of the affinity of whole cells for various energy substrates.
AUTOR(ES)
Belaich, A
RESUMO
Microcalorimetry has been used to determine the affinity of whole cells of Escherichia coli for glucose, galactose, fructose, and lactose. Anaerobic growth thermograms were analyzed, and the Km and Vmax values for these energy substrates were measured at pH 7.8. Results obtained with this technique using various organisms growing anaerobically on different sugars are compared. This comparison shows that in practically all cases the cellular rate of catabolic activity is a hyperbolic function of the energy substrate concentrations at low sugar concentrations. In some cases this technique also allows determination of kinetics at high sugar concentrations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=233330Documentos Relacionados
- Microcalorimetric study of the anaerobic growth of Escherichia coli: growth thermograms in a synthetic medium.
- Microcalorimetric Determination of the Affinity of Saccharomyces cerevisiae for Some Carbohydrate Growth Substrates
- Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.
- Microcalorimetric study of Escherichia coli aerobic growth: theoretical aspects of growth on succinic acid.
- AMMONIA PRODUCTION FROM VARIOUS SUBSTRATES BY PREVIOUSLY PRESSURIZED CELLS OF ESCHERICHIA COLI