Minute virus of mice infection modifies cellular transcription elongation.
AUTOR(ES)
Krauskopf, A
RESUMO
Our previous observations indicated that upon infection with minute virus of mice (MVM), Ehrlich ascites cells lose a transcription elongation activity which is essential for the readthrough of the MVM attenuator. This was monitored by the ability of extracts from uninfected but not from infected cells to support readthrough of the P4 attenuator when added to partially purified transcription elongation complexes. We have investigated the nature of this change in transcription elongation following MVM infection. In this communication, we show that infection of Ehrlich ascites cells with MVM leads to a general shift in the length of nascent mRNA synthesized in isolated nuclei and separated by sucrose gradients. Furthermore, infection leads to attenuation of transcription of the cellular gene c-fos but not c-myc. We show biochemical evidence to support a model by which, following MVM infection, there is a functional reduction in the activity of a TFIIS-like general transcriptional elongation activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236753Documentos Relacionados
- The block to transcription elongation at the minute virus of mice attenuator is regulated by cellular elongation factors.
- A cellular repressor regulates transcription initiation from the minute virus of mice P38 promoter.
- Dissection of transcription factor TFIIF functional domains required for initiation and elongation.
- Nonstructural protein of parvoviruses B19 and minute virus of mice controls transcription.
- Transcription of minute virus of mice, an autonomous parvovirus, may be regulated by attenuation.