Mode of Action of Melinacidin, an Inhibitor of Nicotinic Acid Biosynthesis
AUTOR(ES)
Reusser, Fritz
RESUMO
Melinacidin, a new antibacterial agent, blocked the synthesis of nicotinic acid and its amide in Bacillus subtilis cells. The inhibitory activity of the agent was reversed by nicotinic acid, its amide, or nicotinamide adenine dinucleotides, but not by l-kynurenine, l-3-hydroxykynurenine, l-hydroxyanthranilic acid, or quinolinic acid. These properties indicated that the antibiotic interferes with the conversion of quinolinic acid to nicotinate ribonucleotide by the enzyme quinolinate phosphoribosyl-transferase. However, the activity of a purified preparation of this enzyme derived from a Pseudomonas strain was not impaired by the antibiotic. This suggested that, in B. subtilis, melinacidin interferes with a reaction which occurs before the formation of quinolinic acid in the biosynthetic pathway leading to nicotinic acid. Failure of quinolinic acid to reverse melinacidin inhibition in B. subtilis cultures might be due to insufficient penetration of the cell membranes by quinolinate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252446Documentos Relacionados
- Mode of Action of Albocycline, an Inhibitor of Nicotinate Biosynthesis
- Mode of Action of Thiolutin, an Inhibitor of Macromolecular Synthesis in Saccharomyces cerevisiae
- Simocyclinone D8, an Inhibitor of DNA Gyrase with a Novel Mode of Action
- Biosynthesis of Nicotinic Acid by Mycobacterium tuberculosis
- NICOTINIC ACID BIOSYNTHESIS BY MYCOBACTERIUM TUBERCULOSIS
