Mode of Action of Thiolutin, an Inhibitor of Macromolecular Synthesis in Saccharomyces cerevisiae
AUTOR(ES)
Jimenez, A.
RESUMO
The sulfur-containing antibiotic thiolutin has been shown to be a potent, reversible inhibitor of the growth of Saccharomyces cerevisiae. Viability was unaffected over the concentration range of 4 to 100 μg/ml. At concentrations as low as 2 μg/ml, the drug inhibited ribonucleic acid (RNA) and protein synthesis in whole cells and spheroplasts. At these low concentrations, protein synthesis continued for a short period of time after RNA synthesis was completely stopped. With higher drug concentrations (greater than 20 μg/ml) protein synthesis was inhibited; concentrations of thiolutin up to 100 μg/ml did not affect translocation or peptide bond formation in cell-free protein-synthesizing systems from yeast. The effect of thiolutin on the activity of partially purified deoxyribonucleic acid-dependent RNA polymerases was examined, and the drug was found to be a potent inhibitor of RNA synthesis in vitro. Inhibition was greatest when the polymerase was preincubated with thiolutin. Several mechanisms are discussed to explain the multiple effects of thiolutin on S. cerevisiae. Since the action of the drug is easily reversed, thiolutin may prove to be of use in studies of various stages of yeast growth.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444489Documentos Relacionados
- Macromolecular Synthesis in Saccharomyces cerevisiae in Different Growth Media
- Macromolecular Synthesis During the Germination of Saccharomyces cerevisiae Spores
- In Vivo Effect of Thiolutin on Cell Growth and Macromolecular Synthesis in Escherichia coli
- Effect of Putative Deoxyribonucleic Acid Inhibitors on Macromolecular Synthesis in Saccharomyces cerevisiae
- Mode of action of the hemin-controlled inhibitor of protein synthesis.
