Modificações oxidativas de lipoproteínas de baixa densidade e seus anticorpos : associação com componentes do transporte reverso de colesterol e com a aterosclerose carotídea em adultos sem doença aterosclerótica estabelecida / Oxidative modification of plasma low density lipoproteins and their antibodies : association with the reverse cholesterol transport and carotid atherosclerosis in adults without established aterosclerotic disease

Paolla Fernanda Cezar Ferreira

Cardiovascular disease (DCV) is the most common cause of death on western societies, affecting both sexes, with atherosclerosis being the most important underlying pathology. Atherosclerosis is accepted worldwide as an immuneinflammatory disease, in which the participation of the immune system is crucial for the beginning of endothelial dysfunction, fatty streak formation, progression of lesion and plaque rupture. The oxidative modification of low density lipoproteins (LDL) is an essential step on the atherosclerotic process, generating oxidized LDL particles (oxLDL), which have chemotactic, cytotoxic and immunogenic properties, leading to the formation of specific antibodies (aboxLDL). Thus, it is very important to understand the metabolic and atherosclerotic repercussions of plasma oxLDL and their antibodies on asymptomatic adults. The aims of this study were to determine the atherogenic effects of oxLDL and aboxLDL and their metabolic and anthropometric determinants. For this study, 107 adult individuals were selected, who were classified in tertiles according to serum values of oxLDL and aboxLDL. The biochemical parameters were analyzed through enzymatic-colorimetric, nephelometric and radiometric methods (CETP, PLTP, LH e LPL). The plasma oxLDL was determined using ELISA (Mercodia) and aboxLDL against full LDL using highly oxidized LDL. EIM was measured by Doppler ultrasonography. We observed a reduction on LH activity on the higher tertile of aboxLDL and a borderline increase on EIM (p ? 0.068). The higher oxLDL tertile presented higher concentrations of C, LDL and apoB100 (p ? 0.001), but also presented reduction on CETP activity (p ? 0.029) and higher cIMT (p ? 0.003). We observed significant correlation (p = 0.041) between aboxLDL and cIMT independent of oxLDL or correlation between oxLDL and cIMT independent of aboxLDL. Similar correlations with cIMT between aboxLDL, oxLDL, oxLDL/apoB100 and oxLDL/LDL-C were observed. Serum titles of aboxLDL and probably its presence in the lesion were independent of concentrations of oxLDL. Multivariate regression analysis revealed apoAI as a predictor of aboxLDL (R2= 3.98), and for oxLDL, the preditors were apoB100 (R2= 23,49), CETP (R2= 1,99), and PLTP (R2= 1,78). The behavior and less atherogenic explanation of these biomarkers by components of the TRC were consistent with the attenuation of carotid atherosclerosis observed in individuals with higher levels of antibodies. However, its similarity to oxLDL marker regarding the association with cIMT and its maximum value reveal a double behavior of aboxLDL regarding atherosclerosis This study encourages further investigations to elucidate the mechanisms involved in the atherogenicity of these biomarkers in asymptomatic adults and in pathological states.

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