Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.
AUTOR(ES)
Knutzon, D S
RESUMO
Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48714Documentos Relacionados
- Temporal and tissue-specific regulation of a Brassica napus stearoyl-acyl carrier protein desaturase gene.
- Stearoyl-Acyl Carrier Protein and Unusual Acyl-Acyl Carrier Protein Desaturase Activities Are Differentially Influenced by Ferredoxin1
- Stearoyl-acyl carrier protein delta 9 desaturase from Ricinus communis is a diiron-oxo protein.
- Sequence of a cDNA from Linum usitatissimum encoding the stearoyl-acyl carrier protein desaturase.
- Nucleotide sequence of a stearoyl-acyl carrier protein desaturase cDNA from developing seeds of rice.
