Molecular analysis of a Neurospora crassa gene expressed during conidiation.

AUTOR(ES)

Roberts, A N

RESUMO

The asexual developmental pathway in the life cycle of the filamentous fungus Neurospora crassa culminates in the formation of spores called conidia. Several clones of genomic Neurospora DNA have been isolated that correspond to mRNA species expressed during conidiation and not during mycelial growth (V. Berlin and C. Yanofsky, Mol. Cell. Biol. 5:849-855, 1985). In this paper we describe the characterization of one of these clones, named pCon-10a. This clone contains two genes, con-10 and con-13, which are induced coordinately during the later stages of conidiation. The two genes are separated by 1.4 kilobases of DNA; they are located on linkage group IV and are transcribed from the same strand of DNA. The molecular organization and sequence of one of these genes, con-10, and its flanking regions are presented. Full-length cDNA clones for con-10 also were isolated and sequenced, and transcription-initiation and polyadenylation sites were defined. The con-10 gene contains an open reading frame interrupted by two small introns and encodes an 86-amino-acid residue polypeptide that is both hydrophilic and weakly acidic. Expression of the con-10 gene in various mutants defective at different stages of conidiation indicates that it plays a role after aerial hyphal development. Possible functions, organization, and regulation of conidiation-specific genes are discussed.

Documentos Relacionados

• Genes expressed during conidiation in Neurospora crassa: characterization of con-8.
• Isolation and characterization of genes differentially expressed during conidiation of Neurospora crassa.
• Rco-3, a Gene Involved in Glucose Transport and Conidiation in Neurospora Crassa
• Molecular analysis of nuc-1+, a gene controlling phosphorus acquisition in Neurospora crassa.
• Blue light induction of conidiation-specific genes in Neurospora crassa.