Molecular analysis of chromosome-mediated gene transfer.

AUTOR(ES)

Scangos, G A

RESUMO

Metaphase chromosomes isolated from a cell line carrying the thymidine kinase (TK) gene of herpes simplex virus type I were used to transform the TK-deficient cell line LMTK- to the TK+ phenotype. Four independent transformants were isolated, all of which expressed virus-specific TK. Each of the four transformant cell lines initially became TK- at a rate of 12% per day. All four transformants possessed multiple copies of the TK gene and in one of the four a rearrangement occurred adjacent to the TK sequences. Stable TK+ derivatives of each line, isolated after prolonged cultivation, retained fewer copies of the TK gene than did their unstable parents. The transferred chromosomal fragment was larger than 17 kilobases in each line.

Documentos Relacionados

• Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer.
• Serial transfer of a human gene to rodent cells by sequential chromosome-mediated gene transfer.
• Chromosome-mediated gene transfer of multidrug resistance.
• Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.
• Number and size of human X chromosome fragments transferred to mouse cells by chromosome-mediated gene transfer.