Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14.
AUTOR(ES)
Callahan, P L
RESUMO
The genomic RNA of human rhinovirus type 14 was cloned in Escherichia coli and the complete nucleotide sequence was determined. The RNA genome is 7212 nucleotides long. A single large open reading frame of 6536 nucleotides was identified, which starts at nucleotide 678 and ends 47 nucleotides from the 3' end of the RNA genome. Comparisons of the specified proteins with those of other picornaviruses showed a striking homology (44-65%) between rhinovirus and poliovirus. The rhinovirus genomic RNA is rich in adenosine (32.1%) and strongly favors an adenosine or uridine in the third position of codons. The predicted map locations of all the rhinovirus structural and non-structural proteins and their proposed proteolytic cleavage sites are described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=397120Documentos Relacionados
- The complete nucleotide sequence of a common cold virus: human rhinovirus 14.
- In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.
- Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
- Binding affinities of structurally related human rhinovirus capsid-binding compounds are related to their activities against human rhinovirus type 14.
- WIN 52035-2 inhibits both attachment and eclipse of human rhinovirus 14.