Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
AUTOR(ES)
Jia, Rui, Nie, Liu-Wang, Wang, Ning, Wang, Jingjing
FONTE
Zoologia (Curitiba)
DATA DE PUBLICAÇÃO
2009-06
RESUMO
The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), showed a high level of transcripts in testis, ovary and kidney, whereas little to no signal was detected in other tissues, which suggests that it may play a role during gametogenesis.
Documentos Relacionados
- Molecular cloning and analysis of Myc modulator 1 (Mm-1) from Bufo gargarizans (Amphibia: Anura)
- Diet and trophic niche of Lithobates catesbeianus (Amphibia: Anura)
- Evolution of erythrocyte morphology in amphibians (Amphibia: Anura)
- Isolation and sequencing of the HMG domain of ten Sox genes from Odorrana schmackeri (Amphibia: Anura)
- Genetic analysis reveals candidate species in the Scinax catharinae clade (Amphibia: Anura) from Central Brazil