Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase.
AUTOR(ES)
Härtlein, M
RESUMO
The gene for Escherichia coli leucyl-tRNA synthetase leuS has been cloned by complementation of a leuS temperature sensitive mutant KL231 with an E.coli gene bank DNA. The resulting clones overexpress leucyl-tRNA synthetase (LeuRS) by a factor greater than 50. The DNA sequence of the complete coding regions was determined. The derived N-terminal protein sequence of LeuRS was confirmed by independent protein sequencing of the first 8 aminoacids. Sequence comparison of the LeuRS sequence with all aminoacyl-tRNA synthetase sequences available reveal a significant homology with the valyl-, isoleucyl- and methionyl-enzyme indicating that the genes of these enzymes could have derived from a common ancestor. Sequence comparison with the gene product of the yeast nuclear NAM2-1 suppressor allele curing mitochondrial RNA maturation deficiency reveals about 30% homology.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339939Documentos Relacionados
- Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
- Peptide mapping of aminoacyl-tRNA synthetases: Evidence for internal sequence homology in Escherichia coli leucyl-tRNA synthetase
- Peptide Mapping of Aminoacyl-tRNA Synthetases: Evidence for Internal Sequence Homology in Escherichia coli Leucyl-tRNA Synthetase
- Sequence and structural similarities between the leucine-specific binding protein and leucyl-tRNA synthetase of Escherichia coli.
- ATP-analogues as substrates for the leucyl-tRNA synthetase from Escherichia coli MRE 600.
