Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe.
AUTOR(ES)
Fukui, Y
RESUMO
We have cloned a ras gene homologue from fission yeast Schizosaccharomyces pombe and determined its nucleotide sequence. A putative coding sequence for 219 amino acids was found. The sequence contained one set of splicing signals: GTAAGT for a donor sequence, ACTAA for a unique sequence found in introns of yeast genes and TAG for an acceptor sequence, indicating the existence of an intron. The amino-terminal one third of the predicted S. pombe ras protein was nearly perfectly homologous and the next one third moderately homologous to those of mammalian ras proteins. The carboxy-terminal one third showed no homology but terminated with a short conserved sequence Cys-X-X-Z (X being a hydrophobic amino acid) as in other ras proteins. The result of Southern analysis of S. pombe DNA under nonstringent hybridization conditions using our clone as a probe indicated that no other closely related gene may be present in the S. pombe genome. The transcript of this gene could be detected by Northern analysis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554243Documentos Relacionados
- Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe.
- Cloning and characterisation of the rad9 DNA repair gene from Schizosaccharomyces pombe.
- Sequence of the ribosomal protein gene KD4 from Schizosaccharomyces pombe.
- Nucleotide sequence of a gene encoding a YPT1-related protein from Schizosaccharomyces pombe.
- Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe.
