Molecular cloning of a potential proteinase activated receptor.
AUTOR(ES)
Nystedt, S
RESUMO
A DNA sequence encoding a G-protein-coupled receptor was isolated from a mouse genomic library. The predicted protein is similar in structure to the thrombin receptor and has a similar activation mechanism. When expressed in Xenopus laevis oocytes, the receptor was activated by low concentrations of trypsin (EC 3.4.21.4) and by a peptide (SLIGRL) derived from the receptor sequence, but was not activated by thrombin (EC 3.4.21.5). Trypsin failed to activate a mutant receptor in which the presumed cleavage site Arg-34-Ser-35 was changed to an Arg-Pro sequence. The agonist peptide (SLIGRL) activated equally well mutant and wild-type receptors. Northern blot analysis demonstrated receptor transcripts in highly vascularized tissues such as kidney, small intestine, and stomach. Because this, to our knowledge, is the second example, besides the thrombin receptor, of a proteolytically activated seven-transmembrane G-protein-coupled receptor, we have provisionally named it proteinase activated receptor 2.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=44781Documentos Relacionados
- Molecular cloning of the rat proteinase-activated receptor 4 (PAR4)
- Molecular cloning of a functional human galanin receptor.
- Molecular cloning of a human immunoglobulin G Fc receptor.
- Molecular cloning and characterization of an adenosine receptor: the A3 adenosine receptor.
- Molecular cloning of a gene encoding the histamine H2 receptor.
