Molecular cloning of infectious viral DNA from ecotropic neurotropic wild mouse retrovirus.
AUTOR(ES)
Jolicoeur, P
RESUMO
Among a mixture of amphotropic and ecotropic murine leukemia viruses (MuLVs) isolated from paralyzed wild mice, only N-tropic ecotropic MuLV, cloned by cell culture techniques, has been shown to induce paralysis after reinjection into susceptible mice (M. B. Gardner, Curr. Top. Microbiol. Immunol. 79:215-239, 1978). The viral DNA genome of one of these neurotropic MuLVs (Cas-Br-E) has been cloned in Charon 21A at the SalI site. One clone, designated NE-8, was studied in more detail. A restriction endonuclease map of this cloned DNA was derived. Cloned viral DNA microinjected into NIH 3T3 cells produced infectious MuLV which was characterized as XC+, ecotropic, and N-tropic. The virus that was recovered after the microinjection of NE-8 DNA was also injected into susceptible SIM.S and NIH Swiss mice and was found to induce lower limb paralysis in these animals. These results make it highly unlikely that other agents (which might have escaped detection and separation from ecotropic MuLV by the techniques previously used) play a role in the etiology of this disease and clearly indicate that the ecotropic MuLV genome harbors sequences responsible for this paralysis. The availability of this clone DNA would now allow us to map these sequences on the genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=256526Documentos Relacionados
- Physical mapping of the paralysis-inducing determinant of a wild mouse ecotropic neurotropic retrovirus.
- Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse.
- Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells.
- Virus-coded DNA endonuclease from avian retrovirus.
- Identification of amino acid residues critical for infection with ecotropic murine leukemia retrovirus.