Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.
AUTOR(ES)
Von Tersch, M A
RESUMO
A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic maps of megacinogenic plasmids pBM309 and pBM113. Limited sequence homology between pBM309 and pBM113 was detected by Southern blot hybridization and was mapped to, at most, a 6.4-kilobase-pair region of pBM309 and a 6.1-kilobase-pair region of pBM113.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215787Documentos Relacionados
- Bacteriocin from Bacillus megaterium ATCC 19213: comparative studies with megacin A-216.
- Cloning of a new low-molecular-weight spore-specific protein gene from Bacillus megaterium.
- Cloning of multiple genes involved with cobalamin (Vitamin B12) biosynthesis in Bacillus megaterium.
- Cloning of the gene for C protein, a low molecular weight spore-specific protein from Bacillus megaterium.
- Biosynthesis of phospholipids in Bacillus megaterium.