Molecular diversity of L-type Ca2+ channel transcripts in human fibroblasts.

AUTOR(ES)

Soldatov, N M

RESUMO

The nucleotide sequence of cDNA encoding the human fibroblast Ca2+ channel of L type (HFCC) has been determined. It is highly homologous to L-type channels previously cloned from rabbit lung and heart as well as from rat brain. At least four sites of molecular diversity were identified in the nucleotide sequence of HFCC. Three of these include regions encoding the transmembrane segments IIS6, IIIS2, and IVS3, which are known to be important for channel gating properties. The positions of these sites correlate with RNA splice sites, indicating that the molecular diversity of the transcripts is a result of alternative splicing. The fourth diversity region is located at the C-terminal region and comprises insertions and deletions. It is suggested that these variations may give rise to multiple subforms of HFCC with altered electrophysiological properties.

Documentos Relacionados

• Structure of the voltage-gated L-type Ca2+ channel by electron cryomicroscopy
• Dissecting the functional role of different isoforms of the L-type Ca2+ channel
• Increased phosphorylation of the neuronal L-type Ca2+ channel Cav1.2 during aging
• Hypoxia inhibits the recombinant alpha 1C subunit of the human cardiac L-type Ca2+ channel.
• Characterization of the high-affinity Ca2+ binding sites in the L-type Ca2+ channel pore in rat phaeochromocytoma cells.