Molecular interactions between the components of the En-I transposable element system of Zea mays
AUTOR(ES)
Gierl, Alfons
RESUMO
The sequence of the Inhibitor element Spm-I8 isolated from the wx-m8 allele has been determined. The element is 2242 bp in length. Its ends can be folded into long stem and loop structures. In a line containing the autonomous En element (wx-m8+En) we have detected a 2.5-kb transcript hybridizing to Spm-I8. A cDNA copy of this En-specific transcript containing 1.2 kb of the 3' end was cloned and its DNA sequence was determined. The 3' half of the cDNA is homologous to Spm-I8 and the region of homology is interrupted by intervening sequences. In the absence of an autonomous En element two chimeric transcripts are observed in the wx-m8 line which are probably initiated at the wx promoter and terminate in the Spm-I8 insertion. In the presence of the En element, these transcripts are suppressed, possibly by a trans-acting function of En, inhibiting transcription read-through into Spm-I8.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554228Documentos Relacionados
- The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays
- Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays
- Activation Tagging Using the En-I Maize Transposon System in Arabidopsis
- Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
- Transcription of transposable element Activator (Ac) of Zea mays L
