Molecular structure of a wheat chromosome end healed after gametocidal gene-induced breakage

AUTOR(ES)

Tsujimoto, H.

FONTE

The National Academy of Sciences of the USA

RESUMO

In the progeny of the monosomic addition line of common wheat, Triticum aestivum, carrying the gametocidal chromosome of Aegilops cylindrica, deletion chromosomes carrying the break point within the nucleolar organizing region of chromosome 1B appeared. Attempts were made to amplify the break points by PCR using primers of telomere and rDNA (rRNA gene). In one deletion line, specific amplification of DNA fragments including the 18S rRNA gene, telomere repeats, and their junction occurred. At the junction of telomere and rRNA gene there was a 31-bp inverted duplication of the rRNA gene. Telomere sequences were initiated from the sequence TAG in the duplication. Between the duplications a small sequence was also inserted. This novel DNA sequence at the break point indicates that the breakage–fusion–bridge cycle(s) took place after the first chromatin breakage by the gametocidal gene.

Documentos Relacionados

• Nuclear Gene-Induced Plastome Mutations in OENOTHERA HOOKERI. I. Genetic Analysis
• Improvement of neurological deficits in 6-hydroxydopamine-lesioned rats after transplantation with allogeneic simian virus 40 large tumor antigen gene-induced immortalized dopamine cells
• Epigenetic Control of Chromosome Breakage at the 5′ End of Paramecium tetraurelia Gene A
• Chromatid and Chromosome Type Breakage-Fusion-Bridge Cycles in Wheat (Triticum Aestivum L.)
• Molecular evidence that chromosome breakage by Ds elements is caused by aberrant transposition.