Molecular techniques for the study and diagnosis of parasite infection

AUTOR(ES)

Tavares, RG, Staggemeier, R, Borges, ALP, Rodrigues, MT, Castelan, LA, Vasconcelos, J, Anschau, ME, Spalding, SM

FONTE

Journal of Venomous Animals and Toxins including Tropical Diseases

DATA DE PUBLICAÇÃO

2011

RESUMO

In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality.

Documentos Relacionados

• Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection.
• Laboratory techniques in the diagnosis of herpes simplex infection.
• Molecular techniques in the diagnosis of human infectious diseases.
• Laboratory techniques in the diagnosis and assessment of hepatitis B virus infection.
• Comparison of conventional and molecular techniques for Trypanosoma vivax diagnosis in experimentally infected cattle