Multidrug resistance of DNA-mediated transformants is linked to transfer of the human mdr1 gene.
AUTOR(ES)
Shen, D W
RESUMO
Mouse NIH 3T3 cells were transformed to multidrug resistance with high-molecular-weight DNA from multidrug-resistant human KB carcinoma cells. The patterns of cross resistance to colchicine, vinblastine, and doxorubicin hydrochloride (Adriamycin; Adria Laboratories Inc.) of the human donor cell line and mouse recipients were similar. The multidrug-resistant human donor cell line contains amplified sequences of the mdr1 gene which are expressed at high levels. Both primary and secondary NIH 3T3 transformants contained and expressed these amplified human mdr1 sequences. Amplification and expression of the human mdr1 sequences and amplification of cotransferred human Alu sequences in the mouse cells correlated with the degree of multidrug resistance. These data suggest that the mdr1 gene is likely to be responsible for multidrug resistance in cultured cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367169Documentos Relacionados
- Expression of hamster P-glycoprotein and multidrug resistance in DNA-mediated transformants of mouse LTA cells.
- Multidrug resistance in Leishmania donovani is conferred by amplification of a gene homologous to the mammalian mdr1 gene.
- Expression of the Thy-1 glycoprotein gene by DNA-mediated gene transfer.
- DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.
- Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer.