Multiplication of Bacteriophage P22 in Penicillin-Induced Spheroplasts of Salmonella typhimurium
AUTOR(ES)
Ray, R. K.
RESUMO
The spheroplasts of Salmonella typhimurium (LT2) prepared by treatment with penicillin were capable of adsorbing phage P22 C1. The normal multiplication of the phage took place, although the burst size was reduced to one-fourth of that in intact cells. Rate of incorporation of 14C-thymidine into spheroplasts was increased severalfold on phage infection. Multiplication of C+ also took place, but no lysogeny could be established in spheroplasts. Furthermore, spheroplasts prepared from cells lysogenized with wild-type phage, LT2 (C+), and a temperature-inducible C2 mutant, LT2(tsC2), were not inducible. Unlike normal cells, both mitomycin C and actinomycin D interfered with the phage multiplication in spheroplasts. The spheroplast system offers great advantages in the study of the synthesis of nucleic acids and proteins in phage-infected LT2.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375968Documentos Relacionados
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