Murine spermatogonial stem cells: targeted transgene expression and purification in an active state

AUTOR(ES)

Giuili, Galicia

FONTE

Oxford University Press

RESUMO

A 400 bp fragment of the spermatogonia-specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP-Cam, and the β1- and α6-integrins characteristic of the stem cell fraction. A 700-fold enrichment in stem cells was determined by the ability of the purified fractions to re-establish spermatogenesis in germ cell-depleted recipient testes.

Documentos Relacionados

• Fidelity of targeted recombination in human fibroblasts and murine embryonic stem cells.
• Spermatogonial stem cells of the testis.
• DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.
• Capacity for stochastic self-renewal and differentiation in mammalian spermatogonial stem cells
• Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells