Mutant carrying deletions in the two simian virus 40 early genes.

AUTOR(ES)

Feunteun, J

RESUMO

We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding sequence (0.20 map unit). dl2194 was a viable mutant, it carried a normal helper function for adenovirus growth on monkey cells, and it displayed the transformation properties of a small-t-antigen-negative single mutant. Therefore, none of the known large-T-antigen functions seemed to be altered by the C-terminal deletion.

Documentos Relacionados

• Simian virus 40 mutant with transposed T-antigen and VP1 genes.
• Simian virus 40 mutants carrying extensive deletions in the 72-base-pair repeat region.
• Simian virus 40 mutants carrying two temperature-sensitive mutations.
• Recombination Between Endogenous and Exogenous Simian Virus 40 Genes. I. Rescue of a Simian Virus 40 Temperature-Sensitive Mutant by Passage in Permissive Transformed Monkey Lines
• Organization and expression of early genes of simian virus 40