Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor.
AUTOR(ES)
Guarente, L P
RESUMO
We have isolated a rifampicin-resistant mutant of Escherichia coli RNA polymerase that restores transcription termination in strains with a defective rho protein. In such strains, the mutant RNA polymerase terminates transcription at normally rho-dependent sites at the end of the trp operon, in bacteriophage lambda, and within the lac operon. In addition, a strain with this mutant RNA polymerase remains viable with an amber mutation in rho, whereas a strain with wild-type RNA polymerase does not. These results suggest that the mutant RNA polymerase can terminate transcription at normally rho-dependent sites in the absence of rho.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411233Documentos Relacionados
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