Mutational analysis of the bacteriophage P2 Ogr protein: truncation of the carboxy terminus.
AUTOR(ES)
Gebhardt, K
RESUMO
The Ogr protein is a 72-residue, zinc-binding transcription factor essential for activation of late gene expression in bacteriophage P2. Analysis of C-terminal truncated proteins generated by stop codon mutagenesis shows that deletion of residues distal to position 51 had negligible effects on Ogr function. More-extensive deletion resulted in unstable products with severely reduced activity. These results, as well as the effects of other mutations in this region, support the idea that the 21 C-terminal residues are not required for transactivation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206935Documentos Relacionados
- Control of bacteriophage P2 gene expression: analysis of transcription of the ogr gene.
- Regulation of bacteriophage P2 late-gene expression: the ogr gene.
- Harvey murine sarcoma virus p21 ras protein: biological and biochemical significance of the cysteine nearest the carboxy terminus.
- Escherichia coli K-12 and B contain functional bacteriophage P2 ogr genes.
- Bacteriophage P2 ogr and P4 delta genes act independently and are essential for P4 multiplication.
