Mutational analysis of the lambda int gene: DNA sequence of dominant mutations.

AUTOR(ES)

Bear, S E

RESUMO

We have combined techniques of genetic and physical mapping with rapid DNA sequence analysis to identify the nucleotide change in lambda int mutations. These mutations define two dominant phenotypic classes: (i) recombination that is partially independent of accessory factors, and (ii) inhibition of wild-type Int by missense or nonsense proteins, i.e., negative complementation.

Documentos Relacionados

• Molecular cloning, DNA structure, and RNA analysis of the arginase gene in Saccharomyces cerevisiae. A study of cis-dominant regulatory mutations.
• Retroregulation of the bacteriophage lambda int gene: limited secondary degradation of the RNase III-processed transcript.
• Functional domains of the floral regulator AGAMOUS: characterization of the DNA binding domain and analysis of dominant negative mutations.
• Genetic analysis of the Kirsten-ras-revertant 1 gene: potentiation of its tumor suppressor activity by specific point mutations.
• DNA sequence of the int-xis-Pi region of the bacteriophage lambda; overlap of the int and xis genes.