Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase.
AUTOR(ES)
Colicelli, J
RESUMO
Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360611Documentos Relacionados
- Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
- Transfected human beta-polymerase promoter contains a ras-responsive element.
- Interaction of the two cytosolic domains of mammalian adenylyl cyclase.
- The 70-kilodalton adenylyl cyclase-associated protein is not essential for interaction of Saccharomyces cerevisiae adenylyl cyclase with RAS proteins.
- Identification of regulatory residues of the yeast adenylyl cyclase.