Mutations Affecting N-Acetylglucosaminidase in Dictyostelium discoideum

AUTOR(ES)
RESUMO

A procedure for screening several thousand clones for alterations in a specific gene product was apapplied to mutagenized cultures of Dictyostelium discoideum. Six strains were recovered which had less N-acetylglucosaminidase (EC 3.2.1.30) than the wild type. In addition, we isolated four strains in which the enzyme was temperature-sensitive. The enzyme from one of these strains had an altered substrate affinity.

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