Negative Regulation of DNA Replication by the Retinoblastoma Protein Is Mediated by Its Association with MCM7
AUTOR(ES)
Sterner, Jacqueline M.
FONTE
American Society for Microbiology
RESUMO
A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor. Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130, also bind MCM7. Protein complexes between Rb and MCM7 were also detected in anti-Rb immunoprecipitates prepared from human cells. The amino-termini of Rb and p130 strongly inhibited DNA replication in an MCM7-dependent fashion in a Xenopus in vitro DNA replication assay system. These data provide the first evidence that Rb and Rb-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=110654Documentos Relacionados
- Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling
- A requirement for MCM7 and Cdc45 in chromosome unwinding during eukaryotic DNA replication
- MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication
- Negative Regulation of Cdc18 DNA Replication Protein by Cdc2
- Mcm2 is a target of regulation by Cdc7–Dbf4 during the initiation of DNA synthesis