New aerobactin-mediated iron uptake system in a septicemia-causing strain of Enterobacter cloacae.
AUTOR(ES)
Crosa, L M
RESUMO
Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear magnetic resonance spectrum and fast-atom bombardment mass spectrometry of the siderophore purified from EK33 confirmed its identity with aerobactin. Bioassay screening of a gene library of total DNA of EK33 led to the isolation of several aerobactin-positive clones. Under conditions of iron limitation, these clones expressed in Escherichia coli a protein of 72 kilodaltons that reacted with antiserum raised against the pColV-K30 74-kilodalton aerobactin receptor, while the original E. cloacae strain synthesized an 85-kilodalton protein which also cross-reacted with the antiserum. Restriction endonuclease analysis of the cloned DNA confirmed the structural differences between the two aerobactin genetic systems.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211648Documentos Relacionados
- Aerobactin-mediated iron uptake by Escherichia coli isolates from human extraintestinal infections.
- Cloning of the aerobactin-mediated iron assimilation system of plasmid ColV.
- Characterization of beta-lactamase induction in Enterobacter cloacae.
- O serotyping scheme for Enterobacter cloacae.
- Infectious discitis caused by Enterobacter cloacae.
