New Hepatitis B Virus of Cranes That Has an Unexpected Broad Host Range
AUTOR(ES)
Prassolov, Alexej
FONTE
American Society for Microbiology
RESUMO
All hepadnaviruses known so far have a very limited host range, restricted to their natural hosts and a few closely related species. This is thought to be due mainly to sequence divergence in the large envelope protein and species-specific differences in host components essential for virus propagation. Here we report an infection of cranes with a novel hepadnavirus, designated CHBV, that has an unexpectedly broad host range and is only distantly evolutionarily related to avihepadnaviruses of related hosts. Direct DNA sequencing of amplified CHBV DNA as well a sequencing of cloned viral genomes revealed that CHBV is most closely related to, although distinct from, Ross' goose hepatitis B virus (RGHBV) and slightly less closely related to duck hepatitis B virus (DHBV). Phylogenetically, cranes are very distant from geese and ducks and are most closely related to herons and storks. Naturally occurring hepadnaviruses in the last two species are highly divergent in sequence from RGHBV and DHBV and do not infect ducks or do so only marginally. In contrast, CHBV from crane sera and recombinant CHBV produced from LMH cells infected primary duck hepatocytes almost as efficiently as DHBV did. This is the first report of a rather broad host range of an avihepadnavirus. Our data imply either usage of similar or identical entry pathways and receptors by DHBV and CHBV, unusual host and virus adaptation mechanisms, or divergent evolution of the host genomes and cellular components required for virus propagation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=140978Documentos Relacionados
- Host specificity of a serum marker for hepatitis B: evidence that "e antigen" has the properties of an immunoglobulin.
- Broad host range of human T-cell leukemia virus type 1 demonstrated with an improved pseudotyping system.
- Broad-Host-Range Agrocin of Agrobacterium tumefaciens
- New plasmid-mediated aminoglycoside adenylyltransferase of broad substrate range that adenylylates amikacin.
- A chimeric avian retrovirus containing the influenza virus hemagglutinin gene has an expanded host range.