Nitric oxide levels in cochlear fluids of guinea pig
AUTOR(ES)
Yonamine, Fernando Kaoru, Onishi, Ektor Tsuneo, Mouro, Margareth Gori, Higa, Elisa Mieko Suemitsu, Cruz, Oswaldo Laércio Mendonça
FONTE
Acta Cir. Bras.
DATA DE PUBLICAÇÃO
2013-05
RESUMO
PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
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