Nitrite Reductase of Nitrosomonas europaea Is Not Essential for Production of Gaseous Nitrogen Oxides and Confers Tolerance to Nitrite
AUTOR(ES)
Beaumont, Hubertus J. E.
FONTE
American Society for Microbiology
RESUMO
A gene that encodes a periplasmic copper-type nitrite reductase (NirK) was identified in Nitrosomonas europaea. Disruption of this gene resulted in the disappearance of Nir activity in cell extracts. The nitrite tolerance of NirK-deficient cells was lower than that of wild-type cells. Unexpectedly, NirK-deficient cells still produced nitric oxide (NO) and nitrous oxide (N2O), the latter in greater amounts than that of wild-type cells. This demonstrates that NirK is not essential for the production of NO and N2O by N. europaea. Inactivation of the putative fnr gene showed that Fnr is not essential for the expression of nirK.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=134999Documentos Relacionados
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