Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni2+

AUTOR(ES)

Polacco, Joseph Carmine

RESUMO

Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH4Cl (20 mM); (b) high levels of MgCl2 (10 mM) or CaCl2 (5-10 mM); (c) low levels of NiSO4 (10−2 mM). Additions of 10−2 mM NiSO4 not only overcome citrate growth inhibition but the resultant growth is usually better than urea-supported growth in basal medium (neither added citrate nor added nickel). In the absence of added citrate, exceedingly low levels of NiSO4 (10−4 mM) strongly stimulate urea-supported growth in suspension cultures.

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