Niveis de glutationa reduzida e atividade da catalase, superoxido dismutase e glicose-6-fosfato desidrogenase em individuos expostos ao vapor de mercurio

AUTOR(ES)
DATA DE PUBLICAÇÃO

1995

RESUMO

Mercury (Hg) is used in different human activities. The primary exposition to this metal has its origin from the ingestion of contaminated food and odontologic amalgam. The occupational exposure is the most frequent via in cantamination. Mercury tightly binds to sulfidric groups (SH) presenting cell membrane proteins, enzymes, and low molecular weight substances such as cistein and gluthation. It can form cavalent bonds with sulphur and acts as an oxidant agent when it binds to erythrocytes. The purpose of this study is to investigate the erythrocyte antioxidative system by measurement of Catalase, Superoxide Dismutase (SOD) , Gluthation (GSH) and Glicose-6-Phosphate Dehydrogenase activities in the following groups: Group 1 (EXPOSED)- 16 workers, 14 men and 2 women, exposed to mercury vapors over a period of 0.5 - 8 (3.37 :t 2.40) years. These individuais had 18-48 (32 :t 8.86) years and presented urinary concentrations of mercury lower than the Limit of Biological Tolerance (LBT) 35 - 1.6 (10.73 :t 9.88) ~g/g creatinin. Group 2 (No-EXPOSED)- 7 (M) workers with 42 - 61 ( 46.42 :t 8.61) years without occupational exposed ave r a period of 6 months. They were previously exposed to Hg for 4 to 10 years and presented urinary concentration of mercury of 5.0-19.1 ~g/g creatinin. Group.3 (Normal controls)- composed by technicians and students, 6 men and 2 women, from the Department of Hematology and Pharmacalogy of the State University of Campinas, with 22 - 45 years. They had dental prothesis containing Hg and were not occupationally exposed to this metal. There were no urinary concentrations of mercury in these individuais. Hematologic analysis was carried out in ali the previous groups besides the detection of Heinz bodies. Our results demonstrate that the activity of SOD in exposed and non-exposed individuais presented the same values of the contrai group. Conversely the Catalase activity demonstrated an increasement in group 1 when compared with group 3 and a significantly augmented when compared to group 2. The levels of GSH were significantly reduced in group 1 and normal in group 2. We conclude that the urinary levels of mercury inside the Limit of Biological Tolerance can affect the erytrocyte antioxidant system

ASSUNTO(S)

enzimas toxicologia industrial eritrocitos

ACESSO AO ARTIGO

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