Nonstructural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase.
AUTOR(ES)
Powell, K L
RESUMO
Herpes simplex virus-induced DNA polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. Thus, DEAE-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify DNA polymerase from herpes simplex virus type 1- and type 2-infected cells. The purified enzyme retained unique features of the herpesvirus-induced DNA polymerase, including a requirement for high salt concentrations for maximal activity, a sensitivity to low phosphonoacetate concentrations, and the capacity to be neutralized by rabbit antiserum to herpesvirus-infected cells. By polyacrylamide gel electrophoresis, the purified DNA polymerase was associated with a virus-induced polypeptide of about 150,000 molecular weight.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515973Documentos Relacionados
- Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein.
- Translational regulation of herpes simplex virus DNA polymerase.
- DNA-binding protein associated with herpes simplex virus DNA polymerase.
- Structure-function studies of the herpes simplex virus type 1 DNA polymerase.
- Purification and characterization of varicella-zoster virus-induced DNA polymerase.