Nucleoid-Independent Identification of Cell Division Sites in Escherichia coli
AUTOR(ES)
Cook, William R.
FONTE
American Society for Microbiology
RESUMO
The mechanism used by Escherichia coli to determine the correct site for cell division is unknown. In this report, we have attempted to distinguish between a model in which septal position is determined by the position of the nucleoids and a model in which septal position is predetermined by a mechanism that does not involve nucleoid position. To do this, filaments with extended nucleoid-free regions adjacent to the cell poles were produced by simultaneous inactivation of cell division and DNA replication. The positions of septa that formed within the nucleoid-free zones after division was allowed to resume were then analyzed. The results showed that septa were formed at a uniform distance from cell poles when division was restored, with no relation to the distance from the nearest nucleoid. In some cells, septa were formed directly over nucleoids. These results are inconsistent with models that invoke nucleoid positioning as the mechanism for determining the site of division site formation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=93591Documentos Relacionados
- Nucleoid partitioning and the division plane in Escherichia coli.
- Cell length, nucleoid separation, and cell division of rod-shaped and spherical cells of Escherichia coli.
- Evidence for polar positional information independent of cell division and nucleoid occlusion
- SOS-independent coupling between DNA replication and cell division in Escherichia coli.
- Nucleoid Condensation and Cell Division in Escherichia coli MX74T2 ts52 After Inhibition of Protein Synthesis