Nucleoprotein Complexes Containing Replicating Simian Virus 40 DNA: Comparison with Polyoma Nucleoprotein Complexes
AUTOR(ES)
Hall, Mark R.
RESUMO
Procedures for isolating nucleoprotein complexes containing replicating polyoma DNA from infected mouse cells were used to prepare short-lived nucleoprotein complexes (r-SV40 complexes) containing replicating simian virus 40 (SV40) DNA from infected monkey cells. Like the polyoma complexes, r-SV40 complexes were only partially released from nuclei by cell lysis but could be extracted from nuclei by prolonged treatment with solutions containing Triton X-100. r-SV40 complexes sedimented faster than complexes containing SV40 supercoiled DNA (SV40 complex) in sucrose gradients, and both types of SV40 nucleoprotein complexes sedimented ahead of polyoma complexes containing supercoiled polyoma DNA (py complex). The sedimentation rates of py complex and SV40 complex were 56 and 61S, respectively, based on the sedimentation rate of the mouse large ribosomal subunit as a marker. r-SV40 complexes sedimented as multiple peaks between 56 and 75S. Sedimentation and buoyant density measurements indicated that protein is bound to all forms of SV40 DNA at about the same ratio of protein to DNA (1-2/1) as was reported for polyoma nucleoproteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356709Documentos Relacionados
- Properties of Nucleoprotein Complexes Containing Replicating Polyoma DNA
- Association of simian virus 40 T antigen with replicating nucleoprotein complexes of simian virus 40.
- Histone modifications in simian virus 40 and in nucleoprotein complexes containing supercoiled viral DNA.
- Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.
- Association of Simian Virus 40 T Antigen with Simian Virus 40 Nucleoprotein Complexes