Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.
AUTOR(ES)
Claudio, T
RESUMO
The nucleotide sequence has been determined of a cDNA clone that codes for the 60,000-dalton gamma subunit of Torpedo californica acetylcholine receptor. The length of the cDNA clone is 2,010 base pairs. The 5' and 3' untranslated regions have respective lengths of 31 and 461 base pairs. Data suggest that the putative polyadenylylation consensus sequence A-A-T-A-A-A may not be required for polyadenylylation of the mRNA corresponding to the cDNA clone described in this study. From the DNA sequence data, the amino acid sequence of the gamma subunit was deduced. The subunit is composed of 489 amino acids giving a molecular mass of 56,600 daltons. The deduced amino acid sequence data also indicate the presence of a 17-amino acid extension or signal peptide on this subunit. From these data, structural predictions for the gamma subunit are made such as potential membrane-spanning regions, possible asparagine-linked glycosylation sites, and the assignment of regions of the protein to the extracellular, internal, and cytoplasmic domains of the lipid bilayer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393538Documentos Relacionados
- Nucleotide and deduced amino acid sequence of the goldfish neural nicotinic acetylcholine receptor alpha-3 subunit.
- Nucleotide and deduced amino acid sequence of the goldfish neural nicotinic acetylcholine receptor beta-2 subunit.
- Nucleotide and deduced amino acid sequence of a gamma subunit of bovine fibrinogen.
- Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica.
- Nucleotide sequence of the mouse muscle nicotinic acetylcholine receptor alpha subunit.