Nucleotide sequence analysis reveals novel features of the phase-variable cytadherence accessory protein HMW3 of Mycoplasma pneumoniae.

AUTOR(ES)

Ogle, K F

RESUMO

The Mycoplasma pneumoniae hmw3 gene was sequenced and its gene product was characterized with the goal of elucidating the functional role of HMW3 as an accessory component in cytadherence. A total of 2,016 bp of the hmw3 locus was sequenced, revealing an open reading frame large enough to encode a 672-amino-acid protein with a molecular weight of 73,725. No consensuslike ribosome binding or promoter sequences were identified. However, hmw3 was flanked by upstream and downstream open reading frames. Gene identity was confirmed by comparing the deduced amino acid sequence with the amino acid sequence obtained directly from N-terminal amino acid sequencing of HMW3. Analysis of the deduced amino acid sequence indicated an acidic pI (4.4), an unusual distribution of charged residues, a high degree of hydrophilicity, and a high proline content for HMW3. M. pneumoniae protein profiles obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) following culturing in the presence of [3H]Pro were consistent with the high Pro content predicted for HMW3 and indicated the same for cytadherence accessory protein HMW1. A discrepancy existed between the predicted size of HMW3 (Mr, 73,725) and the size of HMW3 obtained by SDS-PAGE (Mr, 140,000). Variable electrophoretic mobility in SDS-PAGE was observed at different acrylamide concentrations for HMW3, indicating that anomalous migration might account for the size discrepancy. The relative mobility of HMW3 was enhanced only slightly in the presence of magnesium acetate, suggesting that the unusual charge distribution might only be partially responsible for the anomalous migration. Secondary structure predictions were dominated by beta-sheets and nonrepetitive turns or coils, suggesting a probable extended, rigid conformation for HMW3. Finally, an unusual, highly acidic domain (residues 180 to 280) which might have particular functional significance relative to the role of HMW3 as a cytadherence accessory protein was identified.

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