Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
AUTOR(ES)
Sutcliffe, J G
RESUMO
I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392861Documentos Relacionados
- Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.
- Formation of supercoiling domains in plasmid pBR322.
- Genetic analysis of the tetA(C) gene on plasmid pBR322.
- Activities of the RNAI and RNAII promoters of plasmid pBR322.
- Characterization of the beta-lactamase promoter of pBR322.
