Nucleotide sequence of the SV40 DNA restriction fragment Hind C-Hap 2.
AUTOR(ES)
Van Heuverswyn, H
RESUMO
We here report the nucleotide sequence of the SV40 DNA fragment Hind C - Hap 2. The fragment was labeled at the 5'-ends by means of polynucleotide kinase and gamma-(32P) ATP and digested with a suitable restriction enzyme. The separated products were then partially degraded with the base-specific reagents dimethyl-sulphate or hydrazine followed by direct analysis on polyacrylamide gel. The Hind C - Hap 2 sequence is 126 base pairs long and one of the three possible reading frames for translation does not contain any termination codon. So, although no protein is known to be encoded by this region, the possibility cannot yet be completely ruled out. The sequence also contains several AT-rich blocks.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=342501Documentos Relacionados
- Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
- Nucleotide sequence of the restriction fragments hind L and hind M of SV40 DNA.
- The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 2. The sequence of the early strand transcript
- Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
- c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.