Nucleotide sequence of the yeast SUC2 gene for invertase.

AUTOR(ES)

Taussig, R

RESUMO

The yeast SUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5' and 3' flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within the SUC2 gene but share the same reading frame. The amino acid sequences predicted for the two forms of invertase from the nucleotide sequence are consistent with the properties of the purified enzymes. Potential sites for glycosylation of the secreted invertase are identified.

Documentos Relacionados

• SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase.
• Mutations affecting the signal sequence alter synthesis and secretion of yeast invertase.
• Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.
• Secretion-defective mutations in the signal sequence for Saccharomyces cerevisiae invertase.
• An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.